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KMID : 0616120210520040251
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Korean Journal of Pharmacognosy
2021 Volume.52 No. 4 p.251 ~ p.256
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Antioxidant Activity of Ethyl acetate Fraction of Oat in Caenorhabditis elegans
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Kwon Kang-Mu
Kim Jun-Hyeong
Yang Jae-Heon
Ki Byeol-Hui
Hwang In-Hyun
Kim Dae-Keun
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Abstract
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Oat, the seeds of Avena sativa L. (Gramineae), is an important dietary staple for people in many countries. Previous studies reported that A. sativa had various pharmacological effects such as anti-inflammatory, antitumor, neurotonic, and antispasmodic activities. In this study, Caenorhabditis elegans model system was used to investigate the antioxidant activity of methanol extract of oat. The ethyl acetate soluble fraction of the oat methanol extract showed the best DPPH radical scavenging activity. The ethyl acetate fraction was measured for the activity of superoxide dismutase (SOD), catalase, and oxidative stress tolerance by using C. elegans along with reactive oxygen species (ROS) level. In addition, to confirm that the regulation of the stress response gene is responsible for the increased stress tolerance of C. elegans treated by the ethyl acetate fraction, SOD-3 expression was measured using GFP-expressing transgenic worm. As a result, the ethyl acetate fraction increased SOD and catalase activities, and decreased ROS accumulation in a dose-dependent manner. In addition, the ethyl acetate fraction-treated CF1553 worm showed higher SOD-3::GFP intensity compared to the control.
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KEYWORD
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Avena sativa, Caenorhabditis elegans, Antioxidant activity, SOD, Catalase
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